| Product Code | 37656 |
| Bead Size | 40-160um |
| Agarose concentration | 4% |
| Flow rate | 1500cm/h* |
| Binding capacity | 50mg h-lgG/ml gel(static); 40mg h-lgG/ml gel (dynamic)** |
| Cleaning | 6M guanidine, pH 10; 50% methanol, 0.5M NaOH, 15min; 0.1 NaOH 30min - 1hr |
| Storage conditions | 20% ethanol, 2-8C |
| Temperature stability range | Ambient |
| Functional ligand | Recombinant Protein A |
| Leaching | Less then 15ng/mg |
| pH stability range | 2-10 |
| Chemical stability | 8M urea, 6M guanidine, ethanol and common buffers |
| Drug master file | U.S. Food & Drug Administration |
*Flow rate determined in a 1.5x5cm column
**Dynamic binding capacity determind on a 1.5x13cm column
-High dynamic binding capacity
-High flow rate
-Cleanable with 15min 0.5M NaOH washes for up to 50 runs (retaining 80% binding efficiency) Immobilized on Sterogene's patented Ultraflow 4 matrix
-Industrial process-ready
-No animal-sourced materials are used in the manufacturing of either raw materials or the finished product
-Protein A Cellthru plus also available
-cGMP manufacturing; Regulatory Support File available
-DMF with USFDA
Protein A is a cell wall protein derived from Staphyloccal aureus that has the ability to bind the Fc region of lgG molecules. Protein A Ultraflow consists of recombinant protein A immobilized to the base matrix (a highly cross-linked 4% agarose), forming a stable thioether linkage that results in excellent gel stability, low ligand leaching, and a high binding capacity for lgG.
Sterogene's Protein A Ultraflow is comparable to some of the highest quality agarose-based recombinant Protein A resins on the market today. And with its high subsistution levels, great flow rates and low leaching, it makes an excellent choice for a new process, or to update your existing Protein A process.
