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RIDASCREEN® Chinolone/Quinolones

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R3133 RIDASCREEN® Chinolone/Quinolones Microtiter plate with 96 wells (12 strips with 8 r call for price none
Introduction:

Intended use:
RIDASCREEN® Chinolone is a competitive enzyme immunoassay for the quantitative analysis of chinolones in egg, beef, pork, sheep, chicken, turkey, fish and shrimp.

  General information:
Gyrase inhibitors are devided into four subgroups. The majority of chinolones
belong to the subgroup of fluorochinolones, which have a fluoro-group attached at
the central ring system, typically at the 6th position. The fluorchinolones belong to
the so called second generation chinolones. Some of these are admitted as antibiotics in veterinary medicine for food producing animals. Fluorchinolones are broad spectrum antibiotics against a lot of bacterial species. They are used frequently in veterinary medicine especially for cattle, pigs and chicken. The usage of fluorchinolone has increased in the past years because large amounts of
fluorchinolones were applied to prevent infectious diseases, especially in chicken,
swine and fish/shrimp farming.
The wide use in food producing animals has generated microbial resistances. This
led to an amendment of Council Regulation (EEC) No 2377/90 and in the
introduction of MRLs (Maximum Residue Limits) for some fluorchinolones.


  Specifications:
Format:  Microtiter plate with 96 wells (12 strips with 8 removable wells each) 
Standards:  0 ppb (zero standard), 0.5 ppb, 1.5 ppb, 3 ppb, 6 ppb,
18 ppb 
Sample preparation: egg, shrimps: homogenization, extraction, centrifugation
meat, fish: homogenization, extraction, centrifugation,
dilution 
Incubation time:  1 h 15 min 
Detection limit: shrimps: 6 ppb
fish: 8 ppb
egg: 9 ppb
meat: 10 ppb

(corresponding to the standard substance)
Cross-reactions: Ciprofloxacin: 100 %
Norfloxacin, Enrofloxacin, Marbofloxacin,
Danofloxacin, Difloxacin, Flumequin, Ofloxacin: > 100 %
Sarafloxacin: 43 %
Oxolinic acid: 24 %


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