Intended use:
RIDASCREEN® Ractopamin is a competitive enzyme immunoassay for the quantitative analysis of ractopamine in urine, meat and liver.
General information:
Ractopamine belongs to the group of β-agonists. It is known that ß-agonists are suitable for use as performance improvers within the field of livestock production. In particular, ractopamine reduces fat, increases average daily weight gain, and improves feed utilization and conversation ratio (FCR) in fattened animals. However, ß-agonists have not been authorized as growth promoters in livestock in the European Union (EU).
In addition to its lipolytic and anabolic effect, ractopamine has also a relaxing effect on non-striated musculature, on which its therapeutic use as antiasthmatic agent is based. It is possible that ractopamine residues, after use in illegal practice, may lead to a risk for consumers.
Specifications:
Format:Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Standards:0 ppt (zero standard), 100 ppt, 300 ppt, 900 ppt,
2700 ppt, 8100 ppt
Sample preparation: urine: without sample preparation
urine (inclusive hydrolysis): hydrolization, extraction,
centrifugation and evaporation
meat, liver: homogenization, extraction, centrifugation,
evaporation and centrifugation
Incubation time:1 h 30 min
Detection limit: buffer (standard dilution medium): approx. 25 ppt
urine: approx. 600 ppt
urine (inclusive hydrolysis): approx. 700 ppt
meat: approx. 200 ppt
liver: approx. 300 ppt
Cross-reactions: Ractopamine: 100 %
Dobutamine: approx. 4.7 %
Mabuterol: approx. 4.4 %
Ritodrine: approx. 1.4 %
Clenpropanol, Cimaterol: < 0.1 %
Cimbuterol: < 0.01 %
Clenpenterol: < 0.01 %
Mapenterol: < 0.01 %
Terbutaline: < 0.01 %
Bromchlorbuterol: < 0.01 %
Salbutamol: < 0.01 %
Clenbuterol: < 0.01 %
Brombuterol: < 0.01 %
Carbuterol: < 0.01 %
