Intended use:
RIDASCREEN® Biotin is a competitive enzyme binding assay for the quantitative
analysis of added biotin in vitaminized food and vitamin products. The enzymatic
sample preparation for milk and milk powder allows the determination of the total
biotin content (added and natural biotin).
For the detection of Biotin in serum samples a special kit is available under
Art. No.: H1601.
General information:
Biotin, also known as vitamin H, is an important nutritional factor and food additive, playing a major role in the metabolism of both humans and animals alike. Biotin acts as a coenzyme and participates in the process of gluconeogenesis, biosynthesis of fatty acids and the metabolism of amino acids. Biotin deficiency in mammals leads to growth inhibition, loss of appetite and reduced utilization of food components. Advanced stages of avitaminosis manifest themselves in hair loss, dermatitis and signs of paralysis. The biotin requirement for adults is between 100 and 200 mg per day.
Specifications:
Format:Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Standards:0 ppt (zero standard), 37 ppt, 111 ppt, 333 ppt, 1000 ppt
Sample preparation: homogenization, (optional digestion with a-amylase)
heating, centrifugation, (optional Ascorbasetreatment),
extraction with papain (milk and milk powder) and dilution
Incubation time:1 h 30 min
Detection limit: milk: approx. 0.5 ppb
milk powder: approx. 5.5 ppb
grain products: approx. 3.7 ppb
vitamin powder etc. : approx. 370 ppt
vitamin juice: approx. 370 ppt
Cross-reactions: Biotin: 100 %
Biotin-4-Amidobenzoat: approx. 89 %
Biocytin: approx. 83 %
