PureCol® -S collagen is provided as a standard for use in assays where ultra-pure collagen is required. PureCol®-S collagen standard is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography. This product is supplied at approximately a 3 mg/ml concentration. The concentration is confirmed by Biuret protein determination assay. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol®-S is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.
PureCol® -S is ideal for using as a collagen standard in controlled testing and assay procedures. PureCol®-S is sterile filtered and is supplied as a ready to use solution.
Parameter, Testing, and Method
|
PureCol®-S Collagen Standard Solution
Catalog # 5015-A
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Sterilization Method
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Filtration
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Form
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Solution
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Package Size
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20 ml
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Storage Temperature
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2-10 °C
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Expiration Date
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Listed on product label and Certificate of Analysis
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Concentration
(Biuret Protein Determination)
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2.9-3.2 mg/mL
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Purity
(collagen concentration)
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> 99.9%
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pH
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Approx. pH 2, 0.01 N HCl
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Gel Time
(Gel Time Assay)
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< 40 minutes
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Fibrillogenesis
(Fibril Formation)
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>0.5 Absorbance Units
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Polyacrylamide Gel Electrophoresis
(SDS – PAGE)
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> 85% collagen containing within alpha, beta, and gamma bands < 15% collagen contained with bands traveling faster than alpha
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Sterility
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No growth
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Endotoxin (LAL)
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< 1.0 EU/ml
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Cell Attachment Assay
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Pass
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Source
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Bovine Hide – Pepsin Extracted
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