Intended use:
RIDASCREEN® Zeranol is a competitive enzyme immunoassay for the quantitative analysis of zeranol in urine.
General information:
The oestrogenic anabolic agent zeranol (a-Zearalanol) is derived from the mycotoxin zearalenone. It is used to increase the average daily live-weight gain in ruminants and to improve the food conversion efficiency. However, this compound has not been authorized as fattening adjuvant in the EU.
The synthetic compound zeranol possesses about three times the estrogenic activity of zearalenone. Besides its anabolic effects, zeranol causes the clinical signs of hyperestrogenism, particularly reduced fertility and disorders in development. It is thus not impossible that zeranol residues in edible tissues, after use in illegal practice, may lead to a risk for consumers.
Specifications:
Format:Microtiter plate (12 strips with 8 removable wells each)
Standards:0 ppt (zero standard), 100 ppt, 300 ppt, 900 ppt,
2700 ppt, 8100 ppt
Sample preparation: hydrolysis and chromatographic purification by means of RIDA® C18 columns (Art. Nr. R2002)
Incubation time:1 h 15 min
Detection limit: urine: approx. 1.5 ppb
(the standard range in consideration of the dilution
factor is 200 - 16200 ppt)220 ng/l (ppt)
Cross-reactions: Zeranol: 100 %
Zearalanone: approx. 2 %
ß-Zearalanol: approx. 4 %
Zearalenone: < 0.5 %
α-Zearalenol: approx. 21 %
ß-Zearalenol: approx. 2.5 %
17ß-Estradiol: < 0.02 %
Clenbuterol: < 0.02 %
Testosterone: < 0.02 %
