Lactose/Sucrose/D-Glucose Assay Kit

The Lactose/Sucrose/D-Glucose assay kit is suitable for the measurement and analysis of sucrose, lactose and D-glucose in flour mixtures and other materials.

Colourimetric method for the determination of Lactose, Sucrose 
and D-Glucose in foodstuffs, beverages and other materials

Principle:
                          (invertase)
(1) Sucrose + H₂O → D-glucose + D-fructose

                  (β-galactosidase)
(2) Lactose + H₂O → D-glucose + D-galactose

                                 (glucose oxidase)
(3) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

                                                                                    (peroxidase)
(4) 2H₂O₂ + p-hydroxybenzoic acid + 4-aminoantipyrine → 
                                                                         quinoneimine + 4H₂O

Kit size:                             100 assays of each
Method:                             Spectrophotometric at 510 nm
Reaction time:                   ~ 60 min
Detection limit:                  100 mg/L
Application examples: 
Flours, beverages, dairy products, milk, foodstuffs containing milk,
cosmetics, pharmaceuticals and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:
Used and accepted in food analysis

Advantages

• Very competitive price (cost per test)
   
• All reagents stable for > 12 months after preparation
   
• Simple format
   
• Very specific
   
• Rapid reaction
   
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
   
• Standard included