Resistant Starch Assay Kit

The Resistant Starch Test kit for the measurement and analysis of resistant starch in plant materials and starch samples.

Colourimetric method for the determination of Resistant Starch 
in cereal products and feeds

Principle:
                              (α-amylase + amyloglucosidase)
(1) Non-resistant starch + H₂O → D-glucose + maltose (trace)

(2) Aqueous ethanol wash + centrifugation to remove D-glucose + 
     maltose

(3) Dissolution of resistant starch pellet in KOH and neutralisation

                                         (α-amylase + amyloglucosidase)
(4) Dissolved resistant starch + H₂O → D-glucose

                               (glucose oxidase)
(5) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

                                                                                    (peroxidase)
(6) 2H₂O₂ + p-hydroxybenzoic acid + 4-aminoantipyrine → 
                                                                         quinoneimine + 4H₂O

Kit size:                           100 assays
Method:                           Spectrophotometric at 510 nm
Reaction time:                  ~ 120 min (plus overnight incubation)
Detection limit:                 2-100% of sample weight
Application examples: 
Plant materials, starch samples and other materials
Method recognition:     
AOAC (Method 2002.02), AACC (Method 32-40.01) and CODEX
(Type II Method)

Advantages

• Very cost effective
   
• All reagents stable for > 2 years after preparation
   
• Only enzymatic kit available
   
• Measures enzyme resistant starch
   
• Simple format
   
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
   
• Standard included