Total Starch (AA/AMG) Assay Kit

The Total Starch (AA/AMG) test kit is used for the measurement and analysis of total starch in cereal flours and food products. This kit now contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0).

Colourimetric method for the determination of Total Starch in 
cereal products, feeds, foodstuffs and other materials

Principle:
                      (α-amylase, 100°C ± DMSO)
(1) Starch granules + H₂O → maltodextrins

                        (amyloglucosidase)
(2) Maltodextrins + H₂O → D-glucose

                                 (glucose oxidase)
(3) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

                                                                                   (peroxidase)
(4) 2H₂O₂ + p-hydroxybenzoic acid + 4-aminoantipyrine → 
                                                                       quinoneimine + 4H₂O

Kit size:                             100 assays
Method:                             Spectrophotometric at 510 nm
Total assay time:                ~ 90 min
Detection limit:                   1-100% of sample weight
Application examples: 
Cereal flours, food products and other materials
Method recognition:     
AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard Method
No. 168), and RACI (Standard Method)

Advantages

• Very competitive price (cost per test)
   
• All reagents stable for > 12 months after preparation
   
• Simple format
   
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
   
• Standard included