The L-Malic Acid test kit is suitable for the measurement and analysis of L-malic acid in grapes, grape juice and wine using the MegaQuant™ colorimeter (measurement at 505 nm). Suitable for white and red wines at all stages of the winemaking process.
Simple colourimetric method for the determination of L-Malic
Acid in foodstuffs, beverages and other materials
Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+
(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate
(diaphorase)
(3) INT + NADH + H+ → NAD+ + INT-formazan
Kit size: 60 assays
Method: Spectrophotometric at 505 nm
Reaction time: ~ 6 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition: Novel method
Advantages
- Novel product, patented technology
- Highly stable reagents (at least three seasons use)
- Very competitive price (cost per test)
- Spectrophotometer / laboratory / expertise not required
- Very simple procedure
- Rapid reaction time (~6 min)
- Standard included
