L-Ascorbic Acid (L-Ascorbate) Assay Kit

For the specific assay of L-ascorbic acid in beverages, meat, flour, dairy and vegetable products. Content:40 assays per kit

Colourimetric method for the determination of L-Ascorbic Acid
in foodstuffs, feed, wine and other materials

Principle:
(5-methylphenazinium methosulphate)
(1) L-Ascorbic acid + R-H₂ + MTT → dehydroascorbate + MTT-formazan + H⁺
(ascorbic acid oxidase)
(2) L-Ascorbic acid + ½O₂ → dehydroascorbate + H₂O

Kit size:                             40 assays (manual) / 400 (microplate) 
                                          / 400 (auto-analyser)
Method:                             Spectrophotometric at 578 nm
Reaction time:                    ~ 8 min
Detection limit:                   0.175 mg/L
Application examples: 
Wine, beer, fruit juices, soft drinks, jam, milk, dairy products 
(e.g. cheese), dietetic foods, baby foods, processed meat, baking
additives, fruit and vegetables (e.g. tomato and potato), 
pharmaceuticals, feed and other materials (e.g. biological cultures,  
samples, etc.)
Method recognition:     
Methods based on this principle have been accepted by MEBAK

Advantages

• Very competitive price (cost per test)
  
• All reagents stable for > 6 months after preparation
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included
  
• Suitable for manual, microplate and auto-analyser formats