Human CD274 (programmed cell death 1 ligand 1) is a cell membrane protein which is involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. Recent data indicated that cancer cells that express PDL1 promote tumor progression through inhibition of PD1-expressing immune effectors. In addition, PDL1 modulates cell-mediated immunity in the infectious disease setting.
Recombinant human CD274 extracellular domain cDNA (19-238 aa, derived from BC074984) was constructed with codon optimization and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal and expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.
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Parameter, Testing, and Method
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CD274, Human, Recombinant
Catalog # 5126
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Quantity
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0.1 mg (100 µg/vial)
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Volume
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0.2 mL
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Concentration
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0.5 mg/mL
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Purity
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>90% as measured by SDS PAGE
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Formulation
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Formulated in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, L-arginine, DTT and Glycerol
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Form
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Solution
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Production Type
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Recombinant – E. coli
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Storage Temperature
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Keep at -20°C for long term storage. Product is stable at 4 °C for at least 30 days
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Shelf Life
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12 months after receipt
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Sterilization Method
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Filtration
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Cell Attachment Activity
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Passes
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Sterility
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No growth
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Accession Number
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NP_054862.1
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Recombinant Protein Sequence
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MASMTGGQQMGRGHHHHHHGNLYFQG^GEFFTVTVPKDLYV
VEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEE
DLKVQHSSYRQRARLLKDQLSLGNAALQITDVKLQDAGVYRC
MISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQA
EGYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRIN
TTTNEIFYCTFRRLDPEENHTAELVIPELPLAHPPNER |
