CD164 (Sialomucins) belongs to a heterogeneous group of secreted or membrane-associated mucins that appear to play two key but opposing roles in vivo: 1) as cytoprotective or anti-adhesive agents and 2) as adhesion receptors. CD164 is a type I integral transmembrane sialomucin that functions as an adhesion receptor. Recombinant CD164 protein may serve as coating matrix protein for studying of hematopoietic stem cell (HSC) functions in vitro.
Full-length of human CD164 extracellular domain cDNA (24 – 162 aa) was constructed with 29 N-terminal T7/His tag and expressed in E. coli as inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified as soluble protein.
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Parameter, Testing, and Method
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CD164, Human, Recombinant
Catalog # 5100
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Quantity
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0.05 mg (50 µg/vial)
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Volume
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0.1 mL
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Concentration
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0.5 mg/mL
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Purity
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>90%
as measured by SDS PAGE
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Formulation
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Formulated in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl,
KCl, EDTA, L-Arginine, DTT and Glycerol.
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Form
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Solution
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Production Type
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Recombinant – E. coli
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Storage Temperature
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-20 °C
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Shelf Life
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12 months after receipt
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Sterilization Method
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Filtration
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Cell Attachment Activity
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Passes
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Sterility
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No growth
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Gene Symbols
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CD164 (endolyn, MUC-24)
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Accession Number
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NP_006007
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Recombinant Protein Sequence
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MASMTGGQQMGRGHHHHHHGNLYFQGGEFDKNTTQHPNVTTLAPI
SNVTSAPVTSLPLVTTPAPETCEGRNSCVSCFNVSVVNTTCFWIE
CKDESYCSHNSTVSDCQVGNTTDFCSVSTATPVPTANSTAKPTVQ
PSPSTTSKTVTTSGTTNNTVTPTSQPVRKSTFD |
