| Product Code | 6700 |
| Bead Size | 60-160um |
| Agarose concentration | 4% |
| Spacer arm | 5 atoms |
| Flow rate | 1500cm/h* |
| Cleaning | 20mM sodium phosphate, pH 6.2, 1mM DTT, 5mM EDTA |
| Storage conditions | 0.1M sodium acetate, 50% glycerol, pH 4.5, 2-8C |
| Reactive group concentration | >30mg/ml |
| Functional Ligand | Papain enzyme |
| Leaching | <0.1ppm |
| pH stability range | 4-9 |
| Chemical stability | Common buffers at pH optimum |
| Drug master file | U.S. Food & Drug Administration |
*Flow rate determined in a 1.5x5cm column
-For F(ab) production via enzymatic cleavage with papain
-High-grade papain withstands up to 50 cleaning cycles with high retained activity
-Very low levels of leaching
-cGMP manufacturing; Regulatory Support File available
-DMF with USFDA
Papain Actigel is made by immobilizing high-grade papain onto Sterogene's Actigel ALD. This ensures an exclusively uniform, stable secondary amine linkage between the reactive monoaldehyde groups of the activated resin and the primary amine groups of papain.
Coupling efficiency is simply a function of loading, so higher papain concentrations are available upon request, if desired.
Papain is a thiol protease that requires a mild reducing environment for activity. Cleavage is carried out in the presence of thiol compounds such as cysteine or mercaptoethanol. Cleavage occurs in the hinge region of the lgG molecule, yeilding F(ab)fragments and F(c) fragments. The F(ab) fragment is monovalent, and therefore cannot simulate antigen crosslinking or precipitation.
