| Product Code | 2703 |
| Bead Size | 60-160um |
| Agarose concentration | 4% |
| Molecular exclusion limit | 20 million Daitons |
| Spacer arm | 12 atoms, hydrophilic |
| Flow rate | 250cm/h* |
| Cleaning | Organic solvents: 50% ethanol, methanol |
| Storage conditions | 20% ethanol, 2-8C |
| Functional group to attach | Thiol containing molecules |
| Reactive group concentration | 30-45umoles/ml resin |
| Temperature stability range | Ambient |
| Functional ligand | Thiopyrdyl |
| Blocking agents | Not applicable |
| Coupling in organic solvents | Yes |
| Coupling in a packed column | Yes |
| pH stability range | 2-8 |
| Coupling conditions | pH 7-10, at 4-22C for 10min to 1hour |
| Chemical stability | 8M urea, 6M guanidine, ethanol and common buffers |
| Drug master file | U.S. Food & Drug Administration |
*Flow rate determined in a 1.5x5cm column
-For reversible coupling of thiol-containing proteins and subsequent selective elution based on relative thiol reactivity
-High subsititution levels: immobilizations can easily reach 20mg immbolizied protein per ml resin
-Utilizes Sterogene's patented Ultraflow 4 matrix
-Cleanable and industrial process-ready -cGMP manufacturing; Regulatory Support File available
-DMF with USFDA
Actigel T is used for the reversible coupling of thiol-containing ligands and/or the purification of thiol-containing molecules. Appropriate ligands for immobilization to Actigel T include small molecules, peptides or proteins.
During the reaction, a thiol-disulfide exchange takes place, attaching the ligand to the resin through a disulfide bond. This bond can then be cleaved by a reducing agent such as mercaptoethanol or dithiothreitol, liberating the ligand.
This strategy is useful when the molecule of interest has a reactive thiol group, while others thiols in the sample are less active. The molecule of interest binds preferentially to Actigel T, leaving other components of the smaple in solution. Unbound proteins are removed in the wash step, after which bound protein/ligand is cleaved form the resin by a reducing agent.
