| Product Code | 2711 |
| Bead Size | 300-500um |
| Agarose concentration | 4% |
| Spacer arm | 5 atoms, hydrophilic |
| Flow rate | 7400cm/hr* |
| Cleaning | 0.15 IM NaOH** |
| Storage Conditions | 20% ethanol, 2-8C |
| Functional to attach | Primary amines |
| Reactive group concentration | >40umoles/ml |
| Coupling effeciencies | >90% |
| Temperature stability range | 2-100 C (wet) |
| Autoclave | Yes |
| Functional ligand | Monoaldehyde |
| Blocking agents | Ethanolamine*** |
| Coupling in organic solvents | Yes |
| Coupling in a packed column | Yes |
| Leaching | <0.1ppm |
| Drug master file | U.S. Food & Drug Administration |
*Measured on a 1.5 X 5cm column
**NaHSO3 must be used in cleaning with NaOH to protect the aldehyde groups. Please see the product instructions for more details.
Activated ALD Cellthru BigBead is an activated resin for immobilizing primary amines from small ligands to large proteins. Convenience and economy are the key advantages of Activated ALD Cellthru BigBead: coupling is simple, rapid and highly efficient. The larger particle size offers another advantage over traditional chromatography media by the increase interlumen space between the beads, allowing for a thicker, less clarified feed stock to be processed without impedance.
During coupling, an instantaneous, reversible Schiff-base reaction takes place between the monoaldehyde groups and the primary amines of the ligand. The Schiff-base is then reduced to a stable secondary amine by the addition of a reducing agent so mild that it cannot reduce intramolecular disulfide bridges. ALD couples both acidic and basic proteins with the same high efficiency, typically >90% due to the high density of stable aldehyde groups.
Coupling is highly reproducible. Hydrolysis or inactivation of the activated groups is the main cause of irreproducible coupling, frequently encountered with traditional coupling chemistries.
Using ALD chemistry, coupling efficiency is simply a function of loading. No hydrolysis or inactivation of active groups takes place during the coupling reaction. This provides a highly reproducible coupling of ligands, critical for both research and manufacturing.
Prepacked Columns and Kits:Activated ALD Cellthru BigBead Prepacked Column
Activated ALD Cellthru BigBead Scale-Up Kit
