Intended use:
RIDASCREEN® Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone residues in cereals, feed, beer, serum and urine.
General information:
The mycotoxin zearalenone is formed by fungi of the genus Fusarium. Zearalenone is a phytohormone which displays, apart from its anabolic properties, mainly estrogenic effects. Because of its estrogenic properties, zearalenone may induce fertility disorders in animals with clinical signs of hyperestrogenism - an aspect of a disease which although reported mainly in hogs, is described in other species such as cow, horse and sheep, too. The potential health risk for man induced by this mycotoxin, which is taken up with foods of vegetable or animal origin, is extensively discussed.
Specifications:
Format:Microtiter plate with 96 wells (12 strips with 8 wells each)
Standards:0 ppt (zero standard), 50 ppt, 150 ppt, 450 ppt,
1350 ppt, 4050 ppt
Sample preparation: cereals and feed: extraction, filtration and dilution
beer: removing of CO2 and dilution
serum and urine: chromatographic purification by means of RIDA® C18 column (Art. No.: R2002)
Incubation time:2 h 30 min
Detection limit: cereals and feed: approx. 1750 ppt
beer: approx. 250 ppt
serum and urine: approx. 50 ppt
Cross-reactions: Zearalenone: 100 %
α-Zearalenol: approx. 41.6 %
Zeranol (Zearalanol): approx. 27.7 %
β-Zearalenol: approx. 13.8 %
Because of the cross-reactivity with the fattening resource zeranol (zearalanol), which is prohibited to use in the EU, the RIDASCREEN® Zearalenon test can be applied for detection of these residues up to a detection limit of 150 ng/l (ppt) in urine.
