Counting Chambers
Counting Chambers from Hausser
These chambers are the finest quality, optically ground, and polished milled glass chambers available. The chamber is diamond etched and has a double improved Neubauer Ruling, which has a worldwide reputation in hospitals and laboratories for unmatched reliability, meeting the most demanding of standards. The standard Hausser blood counting chambers are one piece construction (measuring 75mmx32mmx4.5mm) ensuring long term durability and absolute accuracy in measurement and count.
We offer both the standard (incline) and the new “V-Load” counting hambers for different charging methods. The tolerances are ( 2% of the volume). Cell Depth: 0.100 mm ( +/-2%); Volume: 0.1 Microliter Ruling; Pattern: Improved Neubauer, 1/400 Square mm Rulings cover 9 square millimeters. Boundary lines of the Neubauer rulings are the center lines of the groups of three. (These are indicated in the illustration ). The central square millimeter is ruled into 25 groups of 16 small squares, each group separated by triple lines, the middle one of which is the boundary. The ruled surface is 0.10 mm below the cover glass, so that the volume over each of 16
Made to Last
In addition to the standard ruling patterns which are etched permanently onto the polished surface, we offer the popular “Bright-Line” counting chamber. This bright-line counting chamber allows the ruling to appear brightly illuminated providing sharp contrast to the bright lines and cells, helping the user with accurate counts and less eyestrain. The triple lines show clearly which cells lie within the counting area and eliminate guesswork.We guarantee that all marks are permanent, and can not be removed even after repeated washings.
Other Chambers and Accessories
In addition to the blood counting chambers, we also offer a variety of other counting chambers and accessories which compliment our discipline.
• The Phase Hemacytometer: used for phase microscopy; it is a one piece brightlined hemacytometer with a reduced thickness.
•The Petroff Hausser Counting Chamber: used for bacteria counting.
•The Sperm Counting Chambers: used primarily in fertility testing; it is offered in a series of depths (10, 20, 40 microns).
•The Howard Mold Counter and the Sedgewick Rafter Counting Chamber are also available as well as a series of hemacytometer and counting chamber cover glasses.
Double Neubauer Ruled Metallized Counting Chamber
An “H” shape moat which forms 2 counting areas are metal plated and double Neubauer ruled providing sharp bright line contrast for consistent cell counts. A “V” cut on each area is used to control the possibility of overflow. Triple dividing lines clearly show cells within the counting area. The ruled surface is 0.1mm below the cover glass; liquid capacity is 0.1mm3/mm2 and 0.00025mm3/grid square.
Ordering: The complete set includes one counting chamber, two 0.5mm cover glasses, one red cell pipette, one white blood cell pipette, tubing, mouthpieces and carrying case.
Etched - Transparent Field with Opaque PatternLevy Double Neubauer
Ruled Hemacytometer
Double improved Neubauer rulings are diamond etched directly into a polished recess to reduce damage from scratches. Opaque surfaces above and below the counting area offer an easy focal reference. The chamber comes with a quick orientation mark for the microscope. 0.1mm cell depth.
Ordering: The complete set includes one counting chamber, two 0.5mm cover glasses, pipettes, and carrying case.
Other Counting Chambers
Fuchs-Rosenthal Counting Chamber:
Designed for counting leukocytes, eosinophils and cells in cerebrospinal fluid. Grid pattern is etched directly onto the glass surface. The chamber is 0.2mm deep
Ordering: Chamber set includes: one chamber and 2 octagonal cover glasses.
Petroff-Hausser Counting Chamber:
New improved Neubauer ruling pattern with 1/400 sq. mm of ruling. Designed for the counting of bacteria, sperm, blood platelets, and the content of vaccines. Cell is 0.2mm deep with 1.5mm thick glass slide allows the use off dark field microscopy.
Nageotte Counting Chamber:
Bright Line double ruling, 0.5mm cell depth.
Palmer, McMaster, Speirs-Levy and Howard Chambers
Leica Bright-Line Counting Chamber
Designed by Leica for accurate counting of blood cells platelets, dust/yeast cluster and specimen samples. Sharp contrast from rhodium plated on “V-Load”. Triple dividing lines clearly delineate counting area for accurate and precise results. Molded from a single piece of thermal and shock resistant glass with a polished chamber and cover glass assuring precise capillary loading and even cell distribution. Fluid volume is 0.1 mm3/mm2 and 0.00025mm2 per each square.
Ordering: The complete set includes: one counting chamber, two 0.4mm cover glass, one red cell pipette, one white cell pipette, tubing, mouthpieces, and storage case
Cellometer™- The Disposable Cell Counting Chamber!!
Cell Counting Made Easy
These Disposable Cell Counters are an enclosed chamber with two ports for sample introduction. The chamber contains precisely spaced lines in a grid pattern. There are two counting chambers per slide for replicates The counting grid pattern is the Improved Neubauer, as in a common Hemacytometer. The consistent pattern design allows the standard cell counting procedure to be followed.
With a pipetor, 15 to 20 microlilter cell-containing sample is introduced into the counting chamber through one of the ports. Liquid spreads inside the chamber, replaces air, which escapes through the opposing port.
The cell counting chamber is placed under a phase contrast optical microscope with a typical magnification of 100. The microscope is focused both onto the grid pattern and the cell particles. Standard protocols are followed for cell counting.
1. Count the total number of cells found in 4 large corner squares (C_total)
2. Cell concentration = Dilution x C_total / Volume
Let the Cellometer™ replace your Hemacytometers, which are traditionally used for cell. Counting!!
Benefits of the Cellometer™:
1.No washing after counting. Extremely important for people whom work with infectious samples.
2.No need to place the cover slide
3.Easy sample introduction. By integrating the cover slide into the counting chamber, the Cellometer™ eliminates the mistakes that may be caused by misplacement of the cover slide or misleading of the sample- two major sources for counting errors when using the Hemacytometer.
4.Made from plastic. Ideal for BioSafety level 3.
EMS Specialty Counting Chambers
• Sedgewick Rafter Cell
• Howard Cell
•Makler Chamber
•Haemocytometry
•Parasitology, Cytology & Others
•Chamber Cover Glasses
This range of quality counting chambers are available in a wide range of types for Haemocytometry, Parasitology, Cytology and other applications
Chambers with inside markings have rulings applied using engraving techniques to give a permanent image, clearly visible through the glass without sacrificing light transmission.
Chambers with ruled cover glass have robust image applied using chrome deposition.
Volume Cell
These are used to create precise volumes of liquid to enable counting of particles in a specified volume of liquid. A thick slide (glass or plastic) has an area cut into its surface that will hold precise volumes of bathing fluid. The shallow well, facilitating easy counting of contained particles. In use, a single drop of liquid is placed on the slide and a coverglass is placed on the platform providing equal and accurate subdivisions of the fluid each of which can be observed for particle counting.
1. Sedgewick Rafter and Cover Glass - S50, S51, S52
For use in water analysis, culture inspection, and any fluid where particles per unit volume need to be determined. Available in two versions:
S50 is an economically priced plastic cell.
S52 is made from glass, with a chromium surface image. This is intended for professional use and when using phase contrast. In each type, a central cell is filled with fluid and a coverglass limits the volume to 1 milliliter. The grid subdivides this into micro-liters.
S51 is the spare cover glass for S50 and S52. Each cell is supplied with one cover glass.
2. Howard
The Howard cell is a glass slide 76mm x 35mm with a central circular island and is used for counting mold fibers and spores in fruit juices, especially from tomatoes. With the K20 coverglass in place a 0.1mm thickness of liquid is contained over the central island. The coverglass has 25 calibrated fields of 1.382mm diameter through which to view the particles. This coverglass removes the necessity of precise adjustment of the microscope and eyepiece graticule in the original Howard method.
3. Makler
For Rapid Semen Analysis – The Makler counting chamber is only 10 microns deep, which is one tenth the depth of other hemacytometers., making it the shallowest of known chambers. This quality chamber is constructed from two pieces of optically flat glass: the first is the chamber, the second is the cover glass, which has a fine grid pattern of 1mm squares and a center area further subdivided into 0.1mm squares. Four quartz pins of precise height hold the cover glass to give an exact trapped specimen depth of 10 microns.
