Sample complexity necessitates extensive fractionation for in-depth proteomic analysis using mass spectrometry. Protein Discovery, now part of the Expedeon group, has developed a commercial system using a novel technology, termed Gel Elution Liquid Fraction Entrapment Electrophoresis (GELFREE), for molecular weight-based fractionation of intact proteins with liquid phase recovery. Eight samples are run in parallel using a programmable control module in ca. 90 minutes. The system is shown to provide robust fractionation over the mass range 3.5 kDa - 500 kDa with high loading capacity, reproducibility, and recovery.
The Gelfree 8100 Fractionation System for mass spectrometry sample preparation partitions complex protein mixtures into userselectable liquid-phase molecular weight fractions.
Additional Information
| Shelf Life |
Cartridges = Minimum 3 Months |
| Storage |
Room Temperature |
Features
• Intact protein molecular weight fractionation, isolation, and purification
• Liquid-phase recovery without band or spot cutting
• Broad mass range fractionation up to 500 kDa
• Up to eight samples processed in parallel
• Programmable fractionation for isolating and purifying targeted proteins
• High protein recovery (>80%)
• High reproducibility (<15% CV)
• Sampling unbiased by hydrophobicity, pI
• High loading capacity (>5X more than a 1D gel)
• Proteins are recovered intact, for complete characterization
Applications
• Simplify and reduce the dynamic range of complex protein mixtures for bottom-up discovery proteomics using LC-MS/MS
• Fractionate and recover proteins intact for top-down proteomics
• Isolate and enrich user-selected molecular weight fractions for targeted protein quantification using LC-MS/MS
• Isolate intact proteins to analyze variants, post-translational modifications, alterations
• Separate protein pull-down components for target protein purification
• Separation, isolation, and intact recovery of antibodies intact for in-depth characterization
Components
The Gelfree 8100 Fractionation System consists of the Gelfree 8100 Fractionation Station and ready-to-use Gelfree 8100 Cartridges.
