Intended use:
RIDASCREEN® 19-Nortestosteron is a competitive enzyme immunoassay for the
quantitative analysis of of 19-nortestosterone in urine.
General information:
The anabolic effects of 19-Nortestosterone are comparable to those of testosterone,
but with less androgenic effects.
In Germany, 19-Nortestosterone-decanoate and -laureate are permitted for veterinary
use. However, the illegal use of 19-Nortestosterone as doping agents in high
performance sports or horse sports are far more common. Some years ago the
increasing application of illegal cocktails as a fattening resource in calf fattening
was reported. Those cocktails usually contained 19-Nortestosterone-ester.
Specifications:
Format:Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Standards:0 ppt (zero standard), 50 ppt, 150 ppt, 500 ppt, 1000 ppt, 3000 ppt
Sample preparation: urine: hydrolysis and chromatographic purification with RIDA® C18 columns (Art. No.: R2002)
Incubation time:1 h 15 min
Detection limit: urine: 3 ppb
(the measuring range in consideration of the dilution
factor is 100 - 6000 ppt)
(detection limit: corresponding to the standard substance)
Cross-reactions: 19-Nortestosterone 17β: 100 %
19-Nortestosterone 17α: approx. 80 %
19-Norethisterone: approx. 74 %
19-Norandrostendione: approx.100 %
18-Methyl-19 NT-17β: approx. 59 %
17α-Ethyl-19 NT-17β: approx. 40 %
15α, 16α-Methyl.-19 NT-17β-acetate: approx. 71 %
Trenbolone: approx. 10 %
17β-Estradiol: approx. 0.1 %
Zeranol: < 0.1 %
DES: < 0.1 %
MPA: < 0.1 %
