Aspartame Assay Kit

The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Aspartame (and breakdown
products) in foodstuffs, beverages and other materials

Principle:
(pH 12.5)
(1) Asp-Phe-O-Me → Asp-Phe + MeOH

(dipeptidase M)
(2) Asp-Phe + H₂O → L-aspartate + L-phenylalanine

(glutamate-oxaloacetate transaminase)
(3) L-Aspartate + 2-oxoglutarate → L-glutamate + oxaloacetate

(L-malate dehydrogenase)
(4) Oxaloacetate + NADH + H⁺ → L-malate + NAD⁺

Kit size:                            50 assays (manual) / 500 (microplate) 
                                         / 500 (auto-analyser)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 5 min
Detection limit:                 0.57 mg/L
Application examples: 
Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic
products, jam, chocolate and other materials
Method recognition:         Novel method 

Advantages

• Very cost effective
  
• All reagents stable for > 12 months after preparation
  
• Only enzymatic kit available
  
• Measures aspartame and breakdown products (L-aspartate and aspartame acid)
  
• Very specific
  
• Very rapid reaction
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included
  
• Suitable for manual, microplate and auto-analyser formats