For the specific measurement of β-amylase in malt flour. Content: 100/200 assays per kit
Colourimetric method for the determination of β-Amylase in
cereal grains, malt, food, beverages and fermentation products
Principle:
(β-amylase)
(1) G3-β-PNP + H2O → G2 + G-β-PNP
(β-glucosidase)
(2) G-β-PNP + H2O → D-glucose + PNP
(alkaline solution)
(3) p-Nitrophenol → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol
Kit size: 100 / 200 assays
Method: Spectrophotometric at 400 nm
Reaction time: ~ 10 min
Detection limit: 0.05 U/mL of sample solution
Application examples:
Cereal flours, malts and other materials
Method recognition:
Modification of RACI (Standard Method)
Advantages
- Very cost effective
- All reagents stable for > 2 years as supplied
- Only enzymatic kit available
- Very specific
- Simple format
- Rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included