Urea/Ammonia (Rapid) Assay Kit

The Urea/Ammonia (Rapid) test kit is suitable for the specific and rapid measurement and analysis of urea and ammonia in water, beverages, milk and food products.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.

UV-method for the determination of Urea and Ammonia in 
foodstuffs, beverages and other materials

Principle:
                      (urease)
(1) Urea + H₂O → 2NH₃ + CO₂

                         (microbial glutamate dehydrogenase)
(2) 2-Oxoglutarate + NADPH + NH₄⁺ → L-glutamic acid + NADP⁺ 
                                                                                   + H₂O

Kit size:                            50 assays of each
Method:                            Spectrophotometric at 340 nm
Reaction time:                   ~ 8 min
Detection limit:                  0.13 mg/L (urea)
                                          0.07 mg/L (ammonia)
Application examples: 
Wine, grape juice, must, fruit juices, soft drinks, milk, cheese, meat,
processed meat, bakery products, seafood, fertilizers, feed,
pharmaceuticals, cosmetics, water (e.g. swimming-pool water), Kjeldahl
analysis, paper (and cardboard) and other materials (e.g. biological 
cultures, samples, etc.)
Method recognition:     
Methods based on this principle have been accepted by NEN and MEBAK

Advantages

• Very rapid reaction due to use of uninhibited glutamate dehydrogenase
   
• Enzymes supplied as stable Suspensions
   
• Very competitive price (cost per test)
   
• All reagents stable for > 2 years after preparation
   
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
   
• Standard included
   
• Extended cofactors stability