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Integrated Total Dietary Fiber Assay Kit

Price(USD):$374.40

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Equl SKU Product Name UOM Specification Units in Stock Price(USD) Add to Cart:
MK-INTDF Integrated Total Dietary Fiber Assay Kit Kit 100 assays per kit 1 $374.40  
Introduction:

The Integrated Total Dietary Fiber test kit is suitable for the measurement and analysis of Integrated Total Dietary Fiber.
Updated to include resistant, branched maltodextrins as a component in dietary fiber.
K-INTDF- An Integrated Procedure (AOAC Method 2009.01 & 2011.25) for the measurement of Total Dietary Fiber (including resistant starch and non-digestible oligosaccharides). This method combines the key attributes of AOAC Official Methods of Analysis 2002.02, 985.29, 991.43, 2001.03.
Specific dietary fiber fractions are measured as follows:
1. Insoluble dietary fiber (IDF), Higher Molecular Weight Soluble Dietary Fiber (SDFP) and Lower Molecular Weight Soluble Dietary Fiber (SDFS) determination (AOAC Method 2011.25)
2. Total High Molecular Weight Dietary Fiber (HMWDF) and SDFS determination (AOAC Method 2009.01)
The enzymes used in these methods are of very high purity; they are effectively devoid of contaminating enzymes active on β-glucan, pectin and arabinoxylan.
Data calculators are located in the Technical Resources tab.

For the determination of Total Dietary Fiber in cereal products, foodstuffs, feeds and other materials. CODEX Type I method (2011)

Principle:
              (Pancreatic α-amylase + amyloglucosidase)
(1) Non-resistant starch + H2O → D-glucose

                      (protease)
(2) Protein + H2O → peptides

(3) IDF (including resistant starch) and alcohol precipitated 
     soluble DF (SDFP) determined gravimetrically


(4) Alcohol soluble DF (SDFS) determined by HPLC

(5) Ash and residual protein determined on DF residues 
     and subtracted

Kit size:                            100 assays
Method:                             Hydrolysis / removal of non-dietary
                                          fibre components
Total assay time:                ~ 3 hr work (over 2 days)
Detection limit:                  0.5-100% of sample weight
Application examples:
Food ingredients, food products and other materials
Method recognition:    
AOAC (Methods 2009.01 and 2011.25), AACC (Method 32-45.01 and
32-50.01) and CODEX (Type I method)

Advantages

  • The only method that is consistent with the CODEX Alimentarius definition of dietary fibre
     
  • High purity / standardised enzymes employed
     
  • All reagents stable for > 2 years
     
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
     
  • Very competitive price (cost per test)
Related Documents
K-INTDF_DATA.pdf:
m.catalog.equl.cn.pdf:
Related Videos

Sample Pre-treatment Format:

Integrated Total Dietary Fiber Assay Kit:


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