Beta Glucan (Yeast & Mushroom) Assay Kit

For the measurement of 1,3:1,6-ß-glucan and α-glucan in yeast preparations. Content: 100 assays per kit

Colourimetric method for the determination of Yeast and
Mushroom β-Glucan in yeast, mushroom, foodstuffs and
other materials

Principle:
(conc. HCl, 30°C, 45 min)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + α-glucan + H₂O →
soluble glucan

(1.3 M HCl, 100°C, 2 h)
(2) Soluble glucan + H₂O → D-glucose + laminarisaccharides (trace)

(exo-1,3-β-glucanase + β-glucosidase)
(3) Laminarisaccharides + H₂O → D-glucose

(glucose oxidase)
(4) D-Glucose + H₂O + O₂ → D-gluconate + H₂O₂

(peroxidase)
(5) 2H₂O₂ + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H₂O

(amyloglucosidase)
(6) α-Glucan + H₂O → D-glucose

Kit size:                            100 assays
Method:                            Spectrophotometric at 510 nm
Total assay time:              ~ 100 min
Detection limit:                 1-100% of sample weight
Application examples: 
Yeast preparations, mushroom preparations and other materials
Method recognition:         Novel method

Advantages

• Very cost effective
  
• All reagents stable for > 12 months after preparation
  
• Only enzymatic kit available
  
• Simple format
  
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  
• Standard included