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Amylose/Amylopectin Assay Kit

Price(USD):$461.11

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Equl SKU Product Name UOM Specification Units in Stock Price(USD) Add to Cart:
MK-AMYL Amylose/Amylopectin Assay Kit Kit 100 assays per kit 9 $461.11  
Introduction:

For the measurement of amylose/amylopectin ratio and content in cereal starches and flours. Based on a Con A precipitation procedure. Content:100 assays per kit

Colourimetric method for the determination of Amylose and 
Amylopectin in cereals, food and feed

Principle:
                                                                      (Con A)
(1) Soluble starch (amylose + amylopectin) → 
                                                        amylose + amylopectin-Con A
                                                        (soluble)         (precipitate)


                          (α-amylase + amyloglucosidase)
(2) Amylose (in solution) + H2O → D-glucose

                                 (glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

                                                                                    (peroxidase)
(4) H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → 
                                                                       quinoneimine + H2O

                                      
                                 (α-amylase + amyloglucosidase)
(5) Total starch (in solution) + H2O → D-glucose

Kit size:                            100 assays
Method:                            Spectrophotometric at 510 nm
Total assay time:               ~ 120 min
Detection limit:                  Amylose 5-95% of total starch content
Application examples:
Cereal starches, flours, pure starches and foods
Method recognition:          Novel method

Advantages

  • Very cost effective (cost per test)
     
  • All reagents stable for > 12 months after preparation
     
  • Only enzymatic kit available
     
  • Accurate and reliable amylose / amylopectin ratio determination 
     
  • Simple format
     
  • Standard included
Related Documents
EK-AMYL Data Booklet.pdf:
EK-AMYL FAQ.pdf:
EK-AMYL Product Performance.pdf:
m.catalog.equl.cn.pdf:
Related Videos

Sample Pre-treatment Format:

Amylose/Amylopectin Assay Kit:


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