The D-Sorbitol/Xylitol test kit is suitable for the measurement and analysis of D-sorbitol and/or xylitol in food products.
Suitable for manual, auto-analyser and microplate formats.
Colourimetric method for the determination of D-Sorbitol and
Xylitol in foodstuffs and wine
Principle:
(sorbitol dehydrogenase)
(1) D-Sorbitol + NAD+ ↔ D-fructose + NADH + H+
(sorbitol dehydrogenase)
(2) Xylitol + NAD+ ↔ D-xylulose + NADH + H+
(diaphorase)
(3) INT + NADH + H+ → NAD+ + INT-formazan
Kit size: 58 assays (manual) / 580 (microplate)
/ 700 (auto-analyser)
Method: Spectrophotometric at 492 nm
Reaction time: ~ 15 min
Detection limit: 0.20 mg/L
Application examples:
Diabetic foods (e.g. honey, jam and chocolate), dietetic foods,
chewing gum, candies, fruit juice (e.g. apple juice), ice-cream, sweets,
bakery products (e.g. desserts), marzipan, paper (and cardboard),
cosmetics, pharmaceuticals and other materials (e.g. biological cultures,
samples, etc.)
Method recognition:
Methods based on this principle have been accepted by IFU and AIJN
Advantages
- Each vial of sorbitol dehydrogenase is stable for > 2 months at 4°C after dissolution
- No wasted diaphorase solution (stable suspension supplied)
- Very competitive price (cost per test)
- Reagents stable for > 2 years as supplied
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual, microplate and auto-analyser formats
