The myo-Inositol Assay Kit is a reliable and accurate enzymatic UV-method for the specific measurement and analysis of myo-inositol in foods, dairy products and various other materials.
Phytic acid content of samples with very low levels of free myo-inositol can also be determined using K-INOSL. This can be achieved by measuring the amount of myo-inositol released after de-phosphorylation of phytic acid with the enzymes phytase and alkaline phosphatase, as used with the Megazyme Phytic Acid/Total Phosphorus Assay Kit (K-PHYT).
Not suitable for the determination of myo-inositol in baby formula.
Colourimetric method for the determination of myo-Inositol
in various sample matrices
Principle:
(myo-inositol dehydrogenase)
(1) myo-Inositol + NAD+ →
2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+
(diaphorase)
(2) INT + NADH + H+ → NAD+ + INT-formazan
Kit size: 50 assays
Method: Spectrophotometric at 492 nm
Reaction time: ~ 10 min
Detection limit: 0.8 mg/L
Application examples:
Animal feeds, food, baby milk formulation and other materials
Method recognition: Novel method
Advantages
- Very cost effective
- All reagents stable for > 2 years after preparation
- Only enzymatic kit available
- Rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
