The Phytic Acid (Total Phosphorus) test kit is a simple method for the measurement and analysis of phytic acid/total phosphorus in food and feed samples. This method does not require purification of phytic acid via anion-exchange chromatography making it amenable to high numbers of samples.
Colourimetric method for the determination of Phytic Acid
in cereal products, seed materials, animal feeds and
other materials
Principle:
(phytase)
(1) Phytic acid + H2O → myo-Inositol (phosphate)n + P i
(alkaline phosphatase)
(2) myo-Inositol (phosphate)n + H2O → myo-inositol + Pi
(3) Pi + ammonium molybdate → 12-molybdophosphoric acid
(diaphorase)
(4) 12-molybdophosphoric acid + H2SO4 / ascorbic acid →
molybdenum blue
Kit size: 50 assays
Method: Spectrophotometric at 655 nm
Reaction time: 25 min enzymic; 1 h for phosphate determination
Detection limit: ~ 11.3 mg phosphorus (~ 40 mg phytic acid)
/100 g material
Application examples:
Seed materials, feeds and foodstuffs
Method recognition: Novel method
Advantages
- Very cost effective
- All reagents stable for > 2 years after preparation
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
