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L-Malic Acid Assay Kit (Analyser Format)

Price(USD):$310.31

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Equl SKU Product Name UOM Specification Price(USD) Add to Cart:
MK-LMALAF L-Malic Acid Assay Kit (Analyser Format) Kit 245.5 mL of prepared reagent (e.g. 1 assay $310.31  
Introduction:

The L-Malic Acid (Analyser Format) test kit is an analyser format for the specific measurement and analysis of L-malic acid (L-malate) in beverages and food products. On calibration, the prepared reagent is linear to > 80 micrograms of L-malic acid per mL of assay solution.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Analyser format UV-method for the determination of L-Malic Acid 
in foodstuffs, beverages and other materials

Principle:
                      (L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+

                         (glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate

Kit size:                            245.5 mL of prepared reagent (R1 + R2)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 3 min
Detection limit:                 20 mg/L (recommended assay)
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:    
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK

Advantages

  • PVP incorporated to prevent tannin inhibition
     
  • Very stable reagent when prepared for auto-analyser applications
     
  • Linear calibration (R2 ~ 0.9994) up to 80 μg/mL of L-malic acid in final reaction solution
     
  • Validated by the University of Wine, Suze la Rousse, France
     
  • Very competitive price (cost per mL of reagent)
     
  • Both enzymes supplied as stable suspensions
     
  • Very rapid reaction (~ 3 min)
Related Documents
EK-LMALAF Data Booklet.pdf:
EK-LMALAF FAQ.pdf:
m.catalog.equl.cn.pdf:
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Sample Pre-treatment Format:

Assay Format for Wine Testing


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