This rapid and reliable manual acetic acid kit is simple to perform (only two absorbance readings required), and because a true end-point is measured, does not involve complicated calculations like other kits. This product is very stable both during storage and use (> 2 years), has extended linearity (compared to ACS based kits), contains PVP to prevent tannin inhibition, and is performed at a relatively low pH (7.4), thus minimising ester hydrolysis related interference. This method is suitable for the measurement of acetic acid/acetate in foods, beverages and other materials. Content:72 assays
Manual format UV-method for the determination of Acetic Acid
in foodstuffs, beverages and other materials
Principle:
(acetate kinase)
(1) Acetic acid + ATP → acetyl-phosphate + ADP
(phosphotransacetylase)
(2) Acetyl-phosphate + CoA → acetyl-CoA + Pi
(pyruvate kinase)
(3) ADP + PEP → ATP + pyruvate
(D-lactate dehydrogenase)
(4) Pyruvate + NADH + H+ → D-lactic acid + NAD+
Kit size: 72 assays (manual) / 720 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 4 min
Detection limit: 0.063 mg/L
Application examples:
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables,
pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products
(and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper
(and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed
and other materials (e.g. biological cultures, samples, etc.)
Method recognition: Improved method
Advantages
- Improved assay format (only two absorbance readings required)
- All reagents stable for > 2 years after preparation
- PVP incorporated to prevent tannin inhibition
- Very rapid reaction (~ 4 min)
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Very competitive price (cost per test)
- Suitable for Manual and Microplate formats
