Kit includes approximately 640 test samples plus controls, contains 5 microtiter plates.
Outbreaks of Avian Influenza (AI) can cause significant losses and are difficult to predict due to the variation in biological characteristics of the virus strain, concurrent infection, environmental stresses, age and sex of the bird, etc. AI is transmitted naturally by wild fowl, although horizontal transmission can occur between infected chicks and susceptible chicks or mature birds. Infection with AI has been associated with a variety of disease syndromes ranging from sub clinical to mild upper respiratory disease to loss of egg production to acute generalized fatal disease1. Antibodies to avian viruses in serum can be detected by the Enzyme Linked Immunosorbent Assay (ELISA)2. The levels of AIV antibodies in serum, as well as the immune status of entire flocks can be monitored using ELISA.3
Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-AIV antibodies present in the serum will bind to the AIV on the microwell forming an AIV antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the AIV antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 - 410 nm with a 630-650 nm reference filter.
