REAGENT FOR DETECTION OF CLASSICAL FEVER VIRUS ON TISSUE SECTION
OR CELL CULTURE
Classical swine fever (CSF) or hog cholera is an extremely contagious disease of swine with high
mortality, especially in young pigs. It is caused by a small 44 nm-in-diameter enveloped virus
belonging to the genus Pestivirus (Flaviviridae family). The CSF virus is transmitted by direct
or indirect contact between animals via the blood, tissues, secretions, and excretions of sick
or dead animals. The routes of infection are ingestion, inhalation, genital infection, and skin
abrasions.
The disease is present across large areas of Asia, Central America, and South America, as well
as in some parts of Europe and Africa. Many countries are free of the disease. The offi cial
diagnosis of classical swine fever currently recognised by the International Offi ce of Epizootics
(OIE) entails isolating the viral strains in a susceptible cell line and identifying them by an immunological
test (direct or indirect immunofl uorescence assay or direct or indirect immunoperoxidase
assay). ELISA tests are also recognised, but only as serological tests.
BIO 272 reacted with all CSFV strains tested (21 strains originated from Belgium, France, Germany,
Switzerland, Austria, The Netherlands, USA, Italy and Czech republic. BIO 272 did not
show any reactivity with 9 BVDV strains tested.
BIO 272 is specifi c for E2 protein.