The protein encoded by human ICAM2 gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene.
Full-length extracellular domain of human ICAM2 gene (25-223 aa) was constructed with 29 N-terminal T7/His tag and expressed in E. coli as inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.
Parameter, Testing, and Method
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ICAM2, Human, Recombinant
Catalog # 5107
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Quantity
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0.1 mg (100 µg/vial)
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Volume
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0.2 mL
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Concentration
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0.5 mg/mL
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Purity
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>90%
as measured by SDS PAGE
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Formulation
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Formulated in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, L-Arginine, DTT and Glycerol.
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Form
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Solution
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Production Type
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Recombinant – E. coli
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Storage Temperature
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-20 °C
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Shelf Life
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12 months after receipt
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Sterilization Method
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Filtration
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Cell Attachment Activity
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Passes
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Sterility
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No growth
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Gene Symbols
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ICAM2 ( CD102 )
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Accession Number
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NP_000864
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Recombinant Protein Sequence
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MASMTGGQQMGRGHHHHHHGNLYFQGGEFELKVFEVHVRPKKLAVEPKGSLEV
NCSTTCNQPEVGGLETSLDKILLDEQAQWKHYLVSNISHDTVLQCHFTCSGKQ
ESMNSNVSVYQPPRQVILTLQPTLVAVGKSFTIECRVPTVEPLDSLTLFLFRGNET
LHYETFGKAAPAPQEATATFNSTADREDGHRNFSCLAVLDLMSRGGNIFHKHSA
PKMLEIYEPVSDSQ |