The protein encoded by human CD276 gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3′ UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29.
Full-length extracellular domain of human CD276 gene (29 – 466 aa) was constructed with 29 N-terminal T7/His tag and expressed in E. coli as inclusion bodies. The final product was refolded using a unique “temperature shift inclusion body refolding” technology and chromatographically purified.
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Parameter, Testing, and Method
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CD276, Human, Recombinant
Catalog # 5123
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Quantity
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0.1 mg (100 µg/vial)
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Volume
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0.2 mL
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Concentration
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0.5 mg/mL
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Purity
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>90%
as measured by SDS PAGE
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Formulation
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Formulated in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl,
KCl, EDTA, L-Arginine, DTT and Glycerol.
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Form
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Solution
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Production Type
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Recombinant – E. coli
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Storage Temperature
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-20 °C
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Shelf Life
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12 months after receipt
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Sterilization Method
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Filtration
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Cell Attachment Activity
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Passes
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Sterility
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No growth
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Gene Symbols
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CD276 (4Ig-B7-H3; B7-H3; B7H3)
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Accession Number
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NP_001019907
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Recombinant Protein Sequence
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MASMTGGQQMGRGHHHHHHGNLYFQGEVQVPEDPVVALVGTDATLCCSFSPEP
GFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLLAQGNASLRLQR
VRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCS
SYQGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSILRVVLGANGTYS
CLVRNPVLQQDAHSSVTITPQRSPTGAVEVQVPEDPVVALVGTDATLRCSFSPEP
GFSLAQLNLIWQLTDTKQLVHSFTEGRDQGSAYANRTALFPDLLAQGNASLRLQR
VRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCS
SYRGYPEAEVFWQDGQGVPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYS
CLVRNPVLQQDAHGSVTITGQPMTFPPEA |
