Human CD14 (monocyte differentiation antigen CD14) is a 375 amino acid, phospholipid anchored cell surface protein. This protein is preferentially expressed on monocytes / macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide.
Full-length extracellular domain of human CD14 gene (20-345 aa) was constructed with 29 N-terminal T7/His tag and expressed in E. coli as inclusion bodies. The final product was refolded using our unique “temperature shift inclusion body refolding” technology and chromatographically purified.
Parameter, Testing, and Method
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CD14, Human, Recombinant
Catalog # 5089
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Quantity
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0.1 mg (100 µg/vial)
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Volume
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0.2 mL
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Concentration
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0.5 mg/mL
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Purity
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>90% as measured by SDS PAGE
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Formulation
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Formulated in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, L-arginine, DTT and Glycerol
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Form
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Solution
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Production Type
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Recombinant – E. coli
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Storage Temperature
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Keep at -20°C for long term storage. Product is stable at 4 °C for at least 30 days
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Shelf Life
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12 months after receipt
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Sterilization Method
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Filtration
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Cell Attachment Activity
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Passes
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Sterility
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No growth
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Accession Number
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NP_000582
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Recombinant Protein Sequence
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MASMTGGQQMGRGHHHHHHGNLYFQGTTPEPCELDDEDFRC
VCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADA
DPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKEL
TLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQ
WLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERG
LMAALCPHKFPAIQNLALRNTGMETPTGVCAALAAAGVQPHSLDL
SHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLR
VLDLSCNRLNRAPQPDELPEVDNLTLDGNPFLVPGTALPHEGSMN |