FibriCol® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available. FibriCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.
FibriCol® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography.
FibriCol® collagen is supplied at approximately a 10 mg/mL concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. FibriCol® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0.
FibriCol® collagen is ideal for applications where high concentrations of collagen are needed. FibriCol® collagen is provided in a 20 mL volume and is contained in user-friendly packaging for use and storage. FibriCol® is sterile filtered and is supplied as a ready to use solution.
|
Parameter, Testing, and Method
|
FibriCol® Collagen Solution
Catalog # 5133-20ML
|
|
Sterilization Method
|
Filtration
|
|
Form
|
Viscous Solution
|
|
Package Size
|
20 mL
|
|
Storage Temperature
|
2-10 °C
|
|
Expiration Date
|
Listed on product label and
Certificate of Analysis
|
|
Concentration
(Biuret Protein Determination)
|
9.0 – 11 mg/mL
|
|
Purity
(collagen concentration)
|
> 99.9%
|
|
pH
|
Approx. pH 2 – 3, 0.01 N HCl
|
|
Gel Time
(Gel Time Assay)
|
< 40 minutes
|
|
Fibrillogenesis
(Fibril Formation)
|
>0.5 Absorbance Units
|
|
Polyacrylamide Gel Electrophoresis
(SDS – PAGE)
|
> 85% collagen containing within alpha, beta, and gamma bans <15% collagen contained with bands traveling faster than alpha
|
|
Sterility
|
No growth
|
|
Endotoxin (LAL)
|
< 1.0 EU/ml
|
|
Source
|
Bovine Hide – Pepsin Extracted
|
Preparation and Usage
Note: Use these recommendations as guidelines to determine the optimal conditions for your culture system.
3-D Gel Preparation Procedure
- Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle mixing/swirling.
- Adjust pH of mixture to 7.2–7.6. Use of sterile 0.1 M NaOH is recommended. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper).
- Adjust final volume to a total of 10 parts with sterile water.
- To prevent gelation, maintain temperature of mixture at 2-10°C.
To form gel, warm to 37°C. For best results allow approximately 90 minutes for gel formation
References Downloads
