PureCol® EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.
PureCol® EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions.
PureCol® EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol® EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.
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Parameter, Testing, and Method
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PureCol® EZ Gel Collagen Solution
Catalog # 5074-G
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Form
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Solution
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Package Size
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35 mL
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Storage Temperature
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2-10 °C
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Expiration Date
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Listed on product label and
Certificate of Analysis
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Concentration
(Biuret Protein Determination)
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˜ 5 mg/ml (0.5%)
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pH
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Approx. pH 6.9 to 7.4
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Gel Time
(Gel Time Assay)
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< 40 minutes
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Sterility
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No growth
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Endotoxin (LAL)
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<1.0 EU/mL
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Cell Attachment Assay
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Pass
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Collagen Source
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Bovine Hide – Pepsin Extracted
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Medium Supplement
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DMEM/F-12 Medium
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L-glutamine Source
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Mixture of L-glutamine and
dipeptide (L-alanine-L-glutamine)
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