PureCol® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available. PureCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.
PureCol® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography. PureCol® collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0. PureCol® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.
PureCol® collagen is provided in a user-friendly packaging for use and storage. PureCol® is sterile filtered and is supplied as a ready to use solution.
Parameter, Testing, and Method
|
PureCol® Collagen Solution
Catalog # 5005-100ml
|
Sterilization Method
|
Filtration
|
Form
|
Solution
|
Package Size
|
100 ml
|
Storage Temperature
|
2-10 °C
|
Expiration Date
|
Listed on product label and
Certificate of Analysis
|
Concentration
(Biuret Protein Determination)
|
2.9-3.2 mg/ml
|
Purity
(collagen concentration)
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> 99.9%
|
pH
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Approx. pH 2, 0.01 N HCl
|
Gel Time
(Gel Time Assay)
|
< 40 minutes
|
Fibrillogenesis
(Fibril Formation)
|
>0.5 Absorbance Units
|
Polyacrylamide Gel Electrophoresis
(SDS – PAGE)
|
> 85% collagen containing within alpha, beta,
and gamma bands < 15% collagen contained with bands traveling faster than alpha
|
Sterility
|
No growth
|
Endotoxin (LAL)
|
< 1.0 EU/ml
|
Osmolality
|
~ 20 mOsm
|
Conductivity
|
~ 2300 µs/cm
|
Cell Attachment Assay
|
Pass
|
Source
|
Bovine Hide – Pepsin Extracted
|
Cell Attachment Bioassay
References Downloads