TeloCol® bovine collagen solution is derived from an acid extraction process yielding atelopeptide-intact collagen. The pro-peptide regions at both ends of the collagen chain, N- and C-telopeptide regions, are maintained.
TeloCol® collagen is approximately 95% Type I collagen with the remainder being comprised of Type III collagen. Each product includes a bottle containing 50 ml of collagen solution accompanied with a bottle of pre-formulated neutralizing solution for the formation of a collagen gel. This product is supplied at a concentration of approximately 3 mg/ml with the concentration for each specific lot provided on the product label and Certificate of Analysis that is available with the purchase of each product. TeloCol® is sterile filtered and provided in user-friendly packaging for use and storage.
TeloCol® is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.
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Parameter, Testing, and Method
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TeloCol®Collagen Solution
Catalog # 5026-50ML
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Sterilization Method
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Filtration
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Form
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Solution
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Package Size
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Collagen -50 ml
Neutralization Solution -10 ml
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Storage Temperature
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2-10 °C
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Expiration Date
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Listed on product label and Certificate of Analysis
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Collagen Concentration
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2.8 – 3.3 mg/ml
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pH
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Approx. pH 2 in 0.01 N HCl
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Gel Time
(Gel Time Assay)
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< 40 minutes
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Fibrillogenesis
(Fibril Formation)
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>0.35 Absorbance Units
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Polyacrylamide Gel Electrophoresis
(SDS – PAGE)
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Characteristic
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Sterility
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No growth
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Endotoxin (LAL)
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< 10.0 EU/ml
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Cell Attachment Assay
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Pass
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Source
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Bovine Hide – Acid Extracted
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Coating Procedure
Note: Employ aseptic practices to maintain the sterility of the product throughout the preparation and handling of the collagen and other solutions.
- Transfer desired volume of collagen solution from the bottle to a dilution vessel if required. Further dilute to desired concentration using sterile 0.01 N HCl solution. Swirl contents gently until material is completely mixed. A typical working concentration may range from 10 to 100 ug/ml. Note: Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
- Add appropriate amount of diluted TeloCol® collagen to the culture surface ensuring that the entire surface is coated.
- Incubate at room temperature, covered, for 1-2 hours. Aspirate any remaining material. Alternatively, incubate at room temperature until surface is dry.
- Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
- Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.
3-D Gel Preparation Procedure using the supplied Neutralization Solution
Note: Employ aseptic practices to maintain the sterility of the product throughout the preparation and handling of the collagen and other solutions.
Note: It is recommended that the collagen and other working solutions be chilled and kept on ice during the preparation of the collagen.
- Determine the desired volume of collagen required.
- Transfer 1 part of chilled neutralization solution into a sterile mixing vessel or tube.
- Transfer 9 parts of the TeloCol® collagen into the sterile mixing vessel or tube for a total of 10 parts.
- Gently agitate the mixture or pipet up and down to mix. Vortexing is not recommended.
- Dispense the collagen mixture in the desired sterile plates or culture vessels.
- Incubate at 37°C for 1 hour for gel formation.
3-D Gel Preparation Procedure without using the supplied Neutralization Solution
Note: It is recommended that the collagen and other working solutions be chilled and kept on ice during the preparation of the collagen.
- Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle swirling.
- Adjust pH of mixture to 7.2–7.6 using sterile 0.1 M NaOH. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper). Mix with gentle swirling.
- Adjust final volume to a total of 10 parts with sterile water and mix.
- To prevent gelation, maintain temperature of mixture at 2–10°C.
- Dispense the TeloCol® collagen mixture in the desired sterile plates or culture vessels.
- To form gel, warm to 37°C. Allow approximately 30 to 60 minutes for gel formation.
Cell Attachment Bioassay
To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37°C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.
