GelCol™ is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.
GelCol™ collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography. The monomer to oligomer ratio content of GelCol™ is slightly lower than the ratio found in PureCol®. GelCol™ collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. GelCol™ is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0. The GelCol™ product is highly purified but undergoes fewer purification steps than the PureCol® product. GelCol™ collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.
GelCol™ collagen is provided in a user-friendly packaging for use and storage. GelCol™ is sterile filtered and is supplied as a ready to use solution.
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Parameter, Testing, and Method
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GelCol™ Collagen Solution
Catalog # 5167-100ML
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Sterilization Method
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Filtration
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Form
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Solution
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Package Size
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100 ml
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Storage Temperature
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2-10 °C
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Expiration Date
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Listed on product label and
Certificate of Analysis
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Concentration
(Biuret Protein Determination)
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2.9-3.2 mg/ml
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Purity
(collagen concentration)
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> 99.9%
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pH
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Approx. pH 2, 0.01 N HCl
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Gel Time
(Gel Time Assay)
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< 40 minutes
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Fibrillogenesis
(Fibril Formation)
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>0.5 Absorbance Units
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Polyacrylamide Gel Electrophoresis
(SDS – PAGE)
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> 85% collagen containing within alpha, beta,
and gamma bands < 15% collagen contained with bands traveling faster than alpha
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Sterility
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No growth
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Endotoxin (LAL)
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< 1.0 EU/ml
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Osmolality
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~ 20 mOsm
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Cell Attachment Assay
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Pass
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Source
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Bovine Hide – Pepsin Extracted
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Coating Procedure
Note:Use these recommendations as guidelines to determine the optimal coating conditions for your culture system.
Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
Dilute Nutragen®in water to ~50 to 100 µg/ml (~1:30). A 0.01 N HCl solution may also be used.
Swirl contents gently until material is completely mixed.
Add appropriate amount of diluted Nutragen® material to the culture surface ensuring that the entire surface is coated.
Incubate at room temperature, covered, for 1-2 hours. Aspirate any remaining material. Alternatively, incubate at room temperature until surface is dry.
Rinse coated surfaces carefully with sterile medium or PBS, avoid scratching surfaces.
Coated surfaces are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.
3-D Gel Preparation Procedure
Slowly add 1 part of chilled 10X PBS or 10X culture media to 8 parts of chilled collagen solution with gentle swirling.
Adjust pH of mixture to 7.2–7.6. Use of sterile 0.1 M NaOH is recommended. Monitor pH adjustment carefully (pH meter, phenol red, or pH paper).
Adjust final volume to a total of 10 parts with sterile water.
To prevent gelation, maintain temperature of mixture at 2-10°C.
To form gel, warm to 37°C. For best results allow approximately 90 to 120 minutes for gel formation.
