α-D-Mannosidase (Bacteroides thetaiotaomicron)

High purity α-D-Mannosidase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.24
CAZy Family: GH92
CAS: 9025-42-7

alpha-mannosidase; alpha-D-mannoside mannohydrolase

Recombinant. From Bacteroides thetaiotaomicron.
In 3.2 M ammonium sulphate.

Specific activity: ~ 8 U/mg protein (on pNP-α-D-mannopyranoside) at pH 6.5 and 40^oC.

Stability: > 4 years at 4^oC.

DESCRIPTION

α-D-Mannosidase (Bacteroides thetaiotaomicron)

EC 3.2.1.24
CAZy Family: GH92
CAS: 9025-42-7

Synonyms:
alpha-mannosidase; alpha-D-mannoside mannohydrolase

Form:
In 3.2 M ammonium sulphate.

Specific activity: 
~ 8 U/mg protein (on pNP-α-D-mannopyranoside) at pH 6.5 and 40^oC.

Stability:
> 4 years at 4^oC.

Unit definition:
One Unit of α-D-Mannosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from 
p-nitrophenyl-α-D-mannopyranoside (5 mM) in MES buffer (100 mM) and 
CaCl₂ (2.5 mM) at pH 6.5 at 40^oC.

Specificity:
Hydrolysis of terminal non-reducing alpha-D-mannose in α-(1-3), α-(1-4) and 
α-(1-6)-D-mannosides.

Applications:
For use in research.