| Product Code | 704 |
| Bead Size | 60-160µm |
| Matrix Structure | Polystyrene |
| Agarose concentration | 4% |
| Molecular exclusion limit | 20 million Daltons |
| Flow rate | 300cm/hr* |
| Cleaning | 1M NaOH |
| Storage Conditions | 20% ethanol, 2-8°C |
| Temperature stability range | 2-100°C (wet) |
| Autoclavable | Hydrated at pH 7 for 30 min |
| pH stability range | 2-14 |
| Chemical stability | 4M KI, 4M NaSCN, 8M urea, 6M guanidine HCl |
| Physical stability | Negligible volume changes due to changes in pH or ionic strength |
| Drug Master File | U.S. Food & Drug Administration |
*Flow rate determined in a 1.5x5cm column
- Low-pressure base matrix for protein applications
- Robust and stable bead for further activation and ligand immobilization
- Low nonspecific binding
- Cleanable with 1M NaOH
- Broad chemical resistance for industrial applications
- Autoclavable
- Cost-effective
- cGMP manufacturing; Regulatory Support File available
- DMF with USFDA
Uniflow 4 is an agarose-based gel filtration medium with bead-shaped particles, suitable for gel filtration or as an affinity chromatography support where low pressures and slow flow rates are needed. Uniflow 4 is best suited to larger molecules like IgG.
Uniflow 4 is a highly stable 4% agarose medium. Its agarose base features a unique polysaccharide composition that resists biological degradation. The Uniflow line of products is available in 4% and 6% agarose concentrations. Additional crosslinking (e.g. Superflow, Ultraflow) greatly enhances the mechanical and chemical stability of the matrix.
Uniflow 4 is an ideal support matrix for immobilizing biologically active substances, as it exhibits very low nonspecific binding. This is especially important when small amounts of biologics need to be isolated by affinity methods (e.g., diagnostic tests) or when a very high degree of purity is required (as with injectable therapeutics).
