Product Code | 11022 |
Bead Size | 300-500µm |
Matrix Structure | 4% agarose, hardened |
Flow rate | 5000cm/h* |
Binding Capacity | 50mg BSA/ml resin |
Cleaning | 0.5M NaOH |
Storage Conditions | 20% ethanol, 2-8C |
Temperature stability range | 0°- 60°C |
Functional ligand | Carboxyl |
Ion Exchanger Type | Weak cation |
Counter ion | Na+ |
pH stability range | 2-14 |
<>> >Chemical stability | Common aqueous, organic, high salt, denaturants |
Physical stability | Negligible volumbe changes due to pH or Ionic state |
Drug master file | U.S. Food & Drug Administration |
*Flow rate determined in a 1.5x5cm column
- Large particle diameter allows crude material to pass through the column with little to no backpressure during direct capture
- Four different ion exchange chemistries can be used with a variety of bead sizes
- Highly robust, chemically stable beads are ideal for process-scale separations
- High product recovery, up to 95%
-cGMP manufacturing; Regulatory Support File available
-DMF with USFDA
Ion exchange Cellthru BigBead Plus resins are made of a hardened agarose matrix with ion exchange groups attached through a spacer arm. Hardening increases the chemical and mechanical stability of the matrix. CM Cellthru BigBead Plus is a large particle, weak cation exchanger where capacity is a function of pH.
Cellthru BigBeads can be used for viscous feed streams and direct capture and also function in standard packed-bed applications using specially manufactured columns. Scale up from pilot to production scale follows a linear relationship, reducing the time spent on method development.