Heparin Actigel

Product Code	37304
Bead Size	40-160µm
Agarose concentration	4%
Spacer Arm	5 atoms, hydrophilic
Flow rate	300cm/h* (higher flow rates available)
Binding Capacity	>10mg ATIII/ml; 120 Units Factor VIII/ml
Cleaning	0.5M NaOH
Storage Conditions	20% ethanol, 2-8C
Cycle Times	100
Temperature stability range	0-60 C
Functional ligand	Porcine heparin
Leaching	<0.1 ppm
pH stability range	2-13
Chemical stability	High salt, 0.1M NaOH (<2hr), 6M guanidine, 70% ethanol
Drug master file	U.S. Food & Drug Administration

*Flow rate determined in a 1.5x5cm column



- Used in a diverse range of applications
- Heparin Actigel is currently employed in multiple FDA-approved therapeutic manufacturing processes
- High binding capacities across the lifetime of the resin (up to 100 cycles)
- Immobilized on Sterogene’s proprietary Uniflow 4 matrix
- Cleanable and industrial process-ready
- The porcine heparin used in Heparin Actigel is itself an approved human therapeutic
- Heparin resins are also available on Superflow, Ultraflow 4, Ultraflow 6 as well as Cellthru 300 and Cellthru BigBead particles
- cGMP manufacturing; Regulatory Support File available
- DMF with USFDA


Purification Applications
- Complement and coagulation factors
- RNA and DNA polymerases
- Restriction endonucleases
- Ligases
- Protein synthesis factors
- Lipases and lipoproteins
- Viral proteins


The Ligand
Heparin is a naturally occurring linear glycosaminoglycan consisting of a repeating dimer of -L-dipyranuronic acid 2-sulfate and 2-deoxy-2-sulphamino--D-glucopyranose 6-sulfate. Heparin exists in a wide range of molecular weights from 5,000-30,000 Daltons. The primary linkages are 1-4 with very little 1-6 branching. Minute amounts of other sugars are also present. Heparin is highly charged and strongly acidic.

Binding and Elution
Immobilized heparin interacts with proteins by two different mechanisms: It can function as an affinity ligand, in which case the protein (e.g. a coagulation factor) is eluted with a buffer containing either salt or heparin. Heparin's anionic sulfate groups also make it capable of functioning as a high-capacity cation exchanger. In this case, the protein is recovered by gradient elution with salt.

With heparin’s unique combination-affinity for many proteins and ion exchange capability- good purification factors can be achieved despite relatively small differences in binding affinities between proteins.

No Detectable Leaching of Heparin
Heparin Actigel is made from our Actigel ALD activated resin. Actigel ALD creates exclusively uniform, stable secondary amine linkages between the reactive monoaldehyde groups and primary amino groups of the heparin.

The stability of the secondary amine is so high that leaching of the heparin is undetectable at 0.1 ppm, the sensitivity limit of the assay. Moreover, Actigel ALD does not release toxic leaving groups; the leaving group during coupling is water.

Easy Sanitization with NaOH
Traditional heparin resins are limited by the fact that they cannot be treated with high concentrations of NaOH, the standard sanitization method for ion exchange and gel filtration media.

In contrast, Sterogene’s heparin media can be sanitized using 0.5M NaOH. About 3-5 bed volumes are required. The resins can also be stored in 0.05M NaOH for extended periods. The stability of the coupled heparin with the stable secondary amine linkage allow these heparin gels to be used in the pH range of 2-13.

Highly Reproducible Resins
Coupling reproducibility problems can arise when the activated groups become inactivated to various degrees during handling and storage (a problem with classical coupling chemistries). Using ALD chemistry for heparin immobilization, coupling efficiency is high and very reproducible because no hydrolysis or inactivation of reactive groups take place during coupling in a side reaction. This provides a highly reproducible resin, well-suited for both research and manufacturing.

Variety of Supports
Heparin Actigel is available on a variety of agarose supports, each with its own applications and advantages. For instance, higher flow rates can be achieved with Heparin Superflow Plus and Heparin Ultraflow 4. Alternatively, Heparin Cellthru 300 and Heparin Cellthru BigBead can be used with viscous and crude feedstreams that are otherwise unsuitable for a standard low particle size (~100 micron) preparative chromatography beads.

Also Consider
Heparin Actigel
Heparin Superflow Plus
Heparin Ultraflow 4
Heparin Ultraflow 6
Heparin Cellthru BigBead

Sterogenes customization services, immobilization services, and Do-It-Yourself kits for resin optimization programs.