Product Code | 37009 |
Bead Size | 60-160µm |
Agarose concentration | 6% |
Flow rate | 1400cm/h* |
Binding Capacity | 10mg GST-tagged protein/ml |
Cleaning | 0.1M NaOH for 30min |
Storage Conditions | 20% ethanol, 2-8C |
Reactive group concentration | 32µmole/ml gel |
Temperature stability range | 0-60 C |
Functional ligand | Glutathione |
pH stability range | 3-12 |
Chemical stability | High salt, 0.1M NaOH (<2hr), 6M guanidine, 70% ethanol |
Drug master file | U.S. Food & Drug Administration |
*Flow rate determined in a 1.5x5cm column
- For binding of GST-tagged proteins
- High binding capacity with low non-specific binding
- Performance is on par with leading immobilized glutathione resins
- Immobilized on Sterogene’s own patented Superflow matrix (Most industry leading tagged fusion protein binding resins use Sterogene’s Superflow matrix.)
- Cleanable and industrial process-ready
- cGMP manufacturing; Regulatory Support File available
- DMF with USFDA
Sterogene manufactures Superflow beads, and has also developed tagged purification beads that perform well compared with the market leaders in tagged protein purification. Superflow is used by many tagged purification resin suppliers today because of its low nonspecific binding properties. These provide effective and efficient one-step purifications in many different expression systems.
Glutathione Affinity Superflow is made by optimized immobilization of high-grade glutathione to Sterogene’s trademarked Superflow 6 matrix. Glutathione/GST purification has proven to be a simple, effective method of purifying recombinant proteins expressed in the pGEX vector. Once bound to the resin, the GST-tagged protein can be eluted with glutathione or the GST can be cleaved from the protein while still on the column, eluting the GST-free recombinant protein.