| Product Code | 2710 |
| Bead Size | 40-160um |
| Agarose concentration | 4% |
| Molecular exclusion limit | 4 million Daitons |
| Spacer arm | 12 atoms, hydrophilic |
| Flow rate | 4000cm/h* |
| Functional group to attach | Thiol, amino, or hydroxyl groups (pH dependent) |
| Functional ligand | Expoxy |
| Coupling in organic solvents | Yes |
| Coupling in a packed column | No |
| Coupling conditions | Dependent on target functional group |
| Drug master file | U.S. Food & Drug Administration |
*Flow rate determined in a 1.5x5cm column
-For immobilizing peptides and small proteins through thiol, amino or hydroxyl
-Multiple immobilization functionality allows more then one type of ligand immobilization
-High flow rates
-High subsititution levels: immobilizations can easily reach 20mg immbolizied protein per ml resin
-Utilizes Sterogene's patented Ultraflow 4 matrix
-cGMP manufacturing; Regulatory Support File available
-DMF with USFDA
Epoxy Activated Ultralfow 4 features epoxy functional groups that react with nucleophilic groups on the ligand. During coupling, the epoxy group is activated by adjusting the pH
Coupling is pH-dependent
-For coupling thiol groups, pH 7-8 (forms a thioether linkage)
-For coupling amino groups, pH 8-10 (forms a stable secondary amine linkage)
-For coupling hydroxyl groups, pH 12 (forms an ether linkage)
This very stable linkage is extremely resistant to hydrolysis.
