| Product Code | 2732 |
| Bead Size | 40-160um |
| Agarose concentration | 4% |
| Spacer arm | 7 atoms, hydrophilic |
| Flow rate | 4500cm/h* |
| Cleaning | High salt, urea, guanidine, 50% ethanol, methanol |
| Storage Conditions | 20% ethanol, 2-8C |
| Functional group to attach | Thiol, amino, or hydroxyl groups (pH dependent) |
| Reactive group concentration | QC, or test |
| Temperature stability range | Ambient |
| Functional ligand | Epibromohydrin |
| Blocking agents | Mercaptoethanol, ethanolamine |
| Coupling in organic solvents | Yes |
| Coupling in a packed column | No |
| Toxicity | None |
| pH stability range | 2-7** |
| Coupling conditions | Dependent on target functional group |
| Chemical stability | 8M urea, 6M guanidine, ethanol and common buffers |
| Drug master file | U.S. Food & Drug Administration |
*Measured on 1.5 x 5cm column
**During Operation. The pH during coupling ranges from 7.5-12.
Actigel B Ultraflow 4 is a reactive resin for covalent immobilization of peptides, carbohydrates, and other ligands containing amino, thiol, or hydroxyl groups. Coupling is pH-dependent:
- For coupling thiol groups: pH 7.5-8.5 (forms thioether linkage)
- For coupling amino groups: pH 8.5-11 (form stable 2o amine linkage)
- For coupling hydroxyl groups: pH 12 (forms ether linkage)
Actigel B Ultraflow 4 features epibromohydrine functional groups that react with nucleophilic groups on the ligand. During coupling, the bromohydrine group is activated by adjusting pH for the desired linkage preference.
