| Product Code | 2721 |
| Bead Size | 40-160µm |
| Agarose concentration | 6% |
| Spacer arm | 5 atoms, hydrophilic |
| Flow rate | 3000cm/hr* |
| Binding capacity | Up to 60mg/ml |
| Cleaning | 0.15 IM NaOH** |
| Storage Conditions | 20% ethanol, 2-8C |
| Functional to attach | Primary amines |
| Reactive group concentration | >50µmoles/ml |
| Coupling effeciencies | >90% |
| Temperature stability range | 2-100 C (wet) |
| Autoclave | Yes |
| Functional ligand | Monoaldehyde |
| Blocking agents | Ethanolamine |
| Coupling in organic solvents | Yes |
| Coupling in a packed column | Yes |
| Leaching | <0.1ppm |
| pH stability range | 2-13 |
| Coupling conditions | pH 3-10, 0-40C, 2-12 hours |
| Chemical stability | 1M NaOh, 8M urea, 6M guanidine, ethanol, and common buffers.. |
| Drug master file | U.S. Food & Drug Administration |
*Measured on a 1.5 X 5cm column
**NaHSO3 must be used in cleaning with NaOH to protect the aldehyde groups. Please see the product instructions for more details.
Actigel ALD Ultraflow 6 is an activated resin used for immobilizing primary amines from small ligands or large proteins, to create a medium-high pressure affinity chromatography matrix. Convenience and economy are the key advantages of Actigel ALD Ultraflow 6: coupling is simple, rapid and highly efficient.
During coupling, an instantaneous, reversible Schiff-base reaction takes place between the monoaldehyde groups and the primary amines of the ligand. The Schiff-base is then reduced to a stable secondary amine by the addition of a reducing agent so mild that it cannot reduce intramolecular disulfide bridges. ALD couples both acidic and basic proteins with the same high efficiency, typically >90% due to the high density of stable aldehyde groups.
Coupling is highly reproducible. Hydrolysis or inactivation of the activated groups is the main cause of irreproducible coupling, frequently encountered with traditional coupling chemistries.
Using ALD chemistry, coupling efficiency is simply a function of loading. No hydrolysis or inactivation of active groups takes place during the coupling reaction. This provides a highly reproducible coupling of ligands, critical for both research and manufacturing.
