Intended use:
Extration and purification of plant DNA from food products.
SureFood® is a modular system consisting of DNA preparation, real-time PCR and PCR-ELISA detection. Each module is separately available.
SureFood® PREP uses the spin column technique. The DNA extract obtained can be used for PCR detection directly without further treatment. Please refer to SureFood® GMO products for DNA detection.
General information:
The world wide production of genetically modified crops (e.g. soya and corn) has steadily increased since the beginning of the 90's. Since the 1st of September 1998 all food products which are containing genetically modified organisms (GMO) must be labeled. As stated by the Council of Ministers of the European Union (EU), regulation for the labeling of Novel Food: Decision Nr. 1139/98/EC. Furthermore since April 2004 the EU directive 1829/2003 came into force and determines the labeling threshold value at 0.9 % GMO for food and feed. In accordance with § 64 of the LFGB (Lebensmittel- und Futtermittelgesetzbuch), the German equivalent of the FDA, R-Biopharm in cooperation with our partner CONGEN offers the solution in accordance with the German official register of investigative protocols presented in the LFGB for a quick and efficient DNA detection of GMOs in various materials. All the way through, from the individual ingredients to the end product.
Specifications:
Format: 2 x 50 reactions
Sample preparation: 1. Homogenization of the basic material and Preheating of the Elution Buffer
2. Lysis at 65 °C
3. Centrifugation of the lysed sample
4. Setting of optimal binding conditions
5. Loading of spin filter and binding of the DNA
6. Washing of the bound DNA
7. Elution of the DNA from the spin filter
Additional Information:
Depending on the expected yield of DNA, the elution buffer volume can be adapted.
Minimum volume for elution is 50 µl. The eluted DNA is ready-to-use for the PCR.
The DNA can be stored for up to 24 hours at 4 °C. For a storage time of
more than 24 hours it should be kept at -20 °C or –70 °C.
Materials required but not provided with the test kit:
• Microcentrifuge
• Eppendorf Thermomixer (for 65 °C) or incubator
• Reaction tubes (1,5 ml)
• Ethanol (99,8 %)
• Pipettes
• Pipette tips with filter (Filter tips), recommended to reduce the risk
of cross contamination
